Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p248 | (1) | WCRB2014

Comparision of DNA fragmentantion and plasma membrane integrity between chilled and frozen semen of bulls

de Mello Papa Patricia , Neto Carlos Ramires , Guasti Priscilla Nascimento , Maziero Rosiara Rosaria Dias , Sancler-Silva Yame F R , Monteiro Gabriel A , Dell'aqua Camila P Freitas , Junqueira Cassio Renesto , Martin Ian , Alvarenga Marco Antonio , Papa Frederico O , Aqua Junior Jose Antonio Dell'

In fixed time artificial insemination (FTAI) is frozen semen is commonly used, however the use of cooled semen is a practice alternative to reduce costs. The aim of this study was compare the difference in DNA fragmentation and plasma membrane integrity between chilled and frozen semen of bulls. Two ejaculates from 20 bulls were collected. Semen was diluted in extender with egg-yolk and glycerol (Botu-Bov™) and divided in two groups: chilled group (CG) samples were cooled...